prehybridization buffer (Thermo Fisher)

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Thermo Fisher prehybridization buffer
Prehybridization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prehybridization+buffer/SSC+buffer/pm41571440-214-6-20
Average 96 stars, based on 1 article reviews

prehybridization buffer - by Bioz Stars, 2026-10

96/100 stars

Images

Membrane:

Article Title: Differential requirement of the transcription factor HOXC13 for the stable maintenance of human papillomavirus genome among high-risk genotypes.
Article Snippet: The viral genome of the high-risk human papillomavirus (HPV), the causative agent of cervical cancer, is stably maintained as extrachromosomal episomes that establish persistent infection.. We previously identified homeobox-transcription factor HOXC13 as an important host protein mediating the short-term retention of the HPV16 and HPV18 genomes in normal human immortalized keratinocytes (NIKS).. Here, we used CRISPR-Cas9 technology to construct HOXC13 knockout (KO) NIKS cells to determine whether HOXC13 is required for the long-term maintenance of high-risk HPV genomes.

Incubation:

Article Title: Two neuropeptide signalings regulate post-mating refusal behavior and reproductive system in male crickets
Article Snippet: After washing with PBS-0.1% Tween-20 (PBT), the TAGs were treated with proteinase K (5 μg/ml; Thermo Fisher Scientific, Waltham, MA, USA) for 10 min at room temperature and refixed using 0.2% glutaraldehyde in 4% PFA/PBS. .. The TAGs were next immersed in prehybridization buffer [50% formamide, 25% 20 x SSC, salmon sperm DNA (1 mg/ml; Thermo Fisher Scientific, Waltham, MA, USA), heparin (1 mg/ml), 0.1% Tween-20] at 50°C for 2 h, and then incubated in the hybridization solution (prehybridization buffer plus RNA probes at the final concentration of 1 ng/μl) at 50°C for 16 h. Next, the washed samples were incubated with the anti-DIG-alkaline phosphatase (AP)-conjugated antibody (Roche, Mannheim, Germany) at 4°C overnight. .. The samples were washed with staining buffer [Tris-HCl (100 mM, pH 8.8), NaCl (100 mM), MgCl 2 (50 mM), 0.1% Tween-20], and then added to nitroblue tetrazolium and bromo- chloro-indolyl-phosphate solution (NBT-BCIP, 1:50 in staining buffer, Roche, Mannheim, Germany) to develop color in the dark.

Article Title: Two neuropeptide signaling pathways regulate post-mating refractoriness and reproductive system in male crickets
Article Snippet: After washing with PBS-0.1% Tween-20 (PBT), the TAGs were treated with proteinase K (5 μg ml − 1 ; Thermo Fisher Scientific, Waltham, MA, USA) for 10 min at room temperature and refixed using 0.2% glutaraldehyde in 4% PFA/PBS. .. The TAGs were next immersed in prehybridization buffer [50% formamide, 25% 20 x SSC, salmon sperm DNA (1 mg ml − 1 ; Thermo Fisher Scientific), heparin (1 mg ml -1 ), 0.1% Tween-20] at 50 °C for 2 h, and then incubated in the hybridization solution (prehybridization buffer plus RNA probes at the final concentration of 1 ng μl − 1 ) at 50°C for 16 h. Next, the washed samples were incubated with the anti-DIG-alkaline phosphatase (AP)-conjugated antibody (Roche) at 4 °C overnight. .. The samples were washed with staining buffer [Tris-HCl (100 mM, pH 8.8), NaCl (100 mM), MgCl 2 (50 mM), 0.1% Tween-20], and then added to nitroblue tetrazolium and bromo-chloro-indolyl-phosphate solution (NBT-BCIP, 1:50 in staining buffer, Roche) to develop color in the dark.

Article Title: Nuclear export of circular RNA.
Article Snippet: Circular RNAs (circRNAs), which are increasingly being implicated in a variety of functions in normal and cancerous cells, are formed by back-splicing of precursor mRNAs in the nucleus. circRNAs are predominantly localized in the cytoplasm, indicating that they must be exported from the nucleus.. Here we identify a pathway that is specific for the nuclear export of circular RNA.. This pathway requires Ran-GTP, exportin-2 and IGF2BP1.

Article Title: Uridine analogs prevent stress granule formation, not by blocking PKR recognition, but by inhibiting the synthesis of T7 RNA Polymerase byproducts.
Article Snippet: .. Membranes were incubated with 50 mL prehybridization buffer [5X SSC, 20 mM Na2HPO4 pH 7.2, 7% SDS, 2X Denhardt’s Solution (Invitrogen), and sheared Salmon Sperm DNA (1 mg per blot)] for at least 1.5 hour at 30°C in a hybridization oven prior to the addition of radiolabeled probes. ..

Article Title: Two neuropeptide signaling pathways regulate post-mating refractoriness and reproductive system in male crickets.
Article Snippet: Afterwashingwith PBS0.1%Tween-20 (PBT), the TAGswere treatedwith proteinase K (5 μgml−1; Thermo Fisher Scientific, Waltham, MA, USA) for 10min at room temperature and refixed using 0.2% glutaraldehyde in 4% PFA/PBS. .. The TAGs were next immersed in prehybridization buffer [50% formamide, 25% 20 x SSC, salmon sperm DNA (1mgml−1; Thermo Fisher Scientific), heparin (1mgml-1), 0.1% Tween-20] at 50 °C for 2 h, and then incubated in the hybridization solution (prehybridization buffer plusRNAprobes at thefinal concentration of 1 ng μl−1) at 50°C for 16 h. Next, the washed samples were incubated with the anti-DIG-alkaline phosphatase (AP)-conjugated antibody (Roche) at 4 °C overnight. .. The samples were washed with staining buffer [Tris-HCl (100mM, pH8.8),NaCl (100mM),MgCl2 (50mM), 0.1% Tween-20], and then added to nitroblue tetrazolium and bromo-chloroindolyl-phosphate solution (NBT-BCIP, 1:50 in staining buffer, Roche) to develop color in the dark.

Hybridization:

Labeling:

other:

Article Title: Sequence grammar and dynamics of subcellular translation revealed by APEX-Ribo-Seq
Article Snippet: Images were acquired using a Zeiss LSM 710 confocal microscope with a 63× objective lens (Zeiss, Plan-Apochromat 63x/1.4 Oil DIC M27) or an Olympus IX83 inverted microscope with a 100× objective lens (Olympus, UPLXAPO100XO) and a CMOS camera ORCA-Fusion (Hamamatsu Photonics, C15939-20U).

Concentration Assay:

Article Title: Allatotropin, DH31, and Proctolin reduce chill tolerance in the two-spotted cricket, Gryllus bimaculatus.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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